La Primera Nacional arrancó oficialmente con el sorteo del fixture de la temporada 2021 después de un show de imitaciones del comediante Milton Ré y un discurso del presidente de la AFA, Claudio Tapia. La hora de las bolillas llegó y el torneo quedó definido con los cruces de los 35 equipos participantes que lucharán por dos ascensos a la máxima categoría.
La competencia estará dividida en dos zonas de 18 y 17 equipos, y los ganadores de cada una jugarán una final para definir el primer premio a la Liga Profesional de Fútbol. Habrá reducido para determinar el segundo ascenso.
| ZONA A | ZONA B | |||||
| FECHA 1 | FECHA 1 | |||||
| LIBRE | vs. | TEMPERLEY | ALL BOYS | vs. | DEP. MORÓN | |
| ESTUDIANTES RC | vs. | DEP. MAIPÚ | GÜEMES (SGO) | vs. | FERRO | |
| GIMNASIA (MZA) | vs. | AGROPECUARIO | ALMAGRO | vs. | TRISTÁN SUÁREZ | |
| DEP. RIESTRA | vs. | QUILMES | SANTAMARINA | vs. | GIMNASIA (JUJUY) | |
| BELGRANO | vs. | TIGRE | DEFENSORES | vs. | INSTITUTO | |
| SAN MARTÍN (TUC) | vs. | ALVARADO | SAN TELMO | vs. | BARRACAS CTRAL. | |
| ALMIRANTE BROWN | vs. | ESTUDIANTES | VILLA DÁLMINE | vs. | IND. RIVADAVIA | |
| NUEVA CHICAGO | vs. | MITRE (SGO) | SAN MARTÍN (SJ) | vs. | ATL RAFAELA | |
| CHACARITA | vs. | ATLANTA | BROWN (ADROGUÉ) | vs. | GUILLERMO BROWN | |
| FECHA 2 | FECHA 2 | |||||
| CHACARITA | vs. | LIBRE | BROWN (ADROGUÉ) | vs. | ALL BOYS | |
| ATLANTA | vs. | NUEVA CHICAGO | GUILLERMO BROWN | vs. | SAN MARTÍN (SJ) | |
| MITRE (SGO) | vs. | ALMIRANTE BROWN | ATL RAFAELA | vs. | VILLA DÁLMINE | |
| ESTUDIANTES | vs. | SAN MARTÍN (TUC) | IND. RIVADAVIA | vs. | SAN TELMO | |
| ALVARADO | vs. | BELGRANO | BARRACAS CTRAL. | vs. | DEFENSORES | |
| TIGRE | vs. | DEP. RIESTRA | INSTITUTO | vs. | SANTAMARINA | |
| QUILMES | vs. | GIMNASIA (MZA) | GIMNASIA (JUJUY) | vs. | ALMAGRO | |
| AGROPECUARIO | vs. | ESTUDIANTES RC | TRISTÁN SUÁREZ | vs. | GÜEMES (SGO) | |
| DEP. MAIPÚ | vs. | TEMPERLEY | FERRO | vs. | DEP. MORÓN | |
| FECHA 3 | FECHA 3 | |||||
| LIBRE | vs. | DEP. MAIPÚ | ALL BOYS | vs. | FERRO | |
| TEMPERLEY | vs. | AGROPECUARIO | DEP. MORÓN | vs. | TRISTÁN SUÁREZ | |
| ESTUDIANTES RC | vs. | QUILMES | GÜEMES (SGO) | vs. | GIMNASIA (JUJUY) | |
| GIMNASIA (MZA) | vs. | TIGRE | ALMAGRO | vs. | INSTITUTO | |
| DEP. RIESTRA | vs. | ALVARADO | SANTAMARINA | vs. | BARRACAS CTRAL. | |
| BELGRANO | vs. | ESTUDIANTES | DEFENSORES | vs. | IND. RIVADAVIA | |
| SAN MARTÍN (TUC) | vs. | MITRE (SGO) | SAN TELMO | vs. | ATL RAFAELA | |
| ALMIRANTE BROWN | vs. | ATLANTA | VILLA DÁLMINE | vs. | GUILLERMO BROWN | |
| NUEVA CHICAGO | vs. | CHACARITA | SAN MARTÍN (SJ) | vs. | BROWN (ADROGUÉ) | |
| FECHA 4 | FECHA 4 | |||||
| NUEVA CHICAGO | vs. | LIBRE | SAN MARTÍN (SJ) | vs. | ALL BOYS | |
| CHACARITA | vs. | ALMIRANTE BROWN | BROWN (ADROGUÉ) | vs. | VILLA DÁLMINE | |
| ATLANTA | vs. | SAN MARTÍN (TUC) | GUILLERMO BROWN | vs. | SAN TELMO | |
| MITRE (SGO) | vs. | BELGRANO | ATL RAFAELA | vs. | DEFENSORES | |
| ESTUDIANTES | vs. | DEP. RIESTRA | IND. RIVADAVIA | vs. | SANTAMARINA | |
| ALVARADO | vs. | GIMNASIA (MZA) | BARRACAS CTRAL. | vs. | ALMAGRO | |
| TIGRE | vs. | ESTUDIANTES RC | INSTITUTO | vs. | GÜEMES (SGO) | |
| QUILMES | vs. | TEMPERLEY | GIMNASIA (JUJUY) | vs. | DEP. MORÓN | |
| AGROPECUARIO | vs. | DEP. MAIPÚ | TRISTÁN SUÁREZ | vs. | FERRO | |
| FECHA 5 | FECHA 5 | |||||
| LIBRE | vs. | AGROPECUARIO | ALL BOYS | vs. | TRISTÁN SUÁREZ | |
| DEP. MAIPÚ | vs. | QUILMES | FERRO | vs. | GIMNASIA (JUJUY) | |
| TEMPERLEY | vs. | TIGRE | DEP. MORÓN | vs. | INSTITUTO | |
| ESTUDIANTES RC | vs. | ALVARADO | GÜEMES (SGO) | vs. | BARRACAS CTRAL. | |
| GIMNASIA (MZA) | vs. | ESTUDIANTES | ALMAGRO | vs. | IND. RIVADAVIA | |
| DEP. RIESTRA | vs. | MITRE (SGO) | SANTAMARINA | vs. | ATL RAFAELA | |
| BELGRANO | vs. | ATLANTA | DEFENSORES | vs. | GUILLERMO BROWN | |
| SAN MARTÍN (TUC) | vs. | CHACARITA | SAN TELMO | vs. | BROWN (ADROGUÉ) | |
| ALMIRANTE BROWN | vs. | NUEVA CHICAGO | VILLA DÁLMINE | vs. | SAN MARTÍN (SJ) | |
| FECHA 6 | FECHA 6 | |||||
| ALMIRANTE BROWN | vs. | LIBRE | VILLA DÁLMINE | vs. | ALL BOYS | |
| NUEVA CHICAGO | vs. | SAN MARTÍN (TUC) | SAN MARTÍN (SJ) | vs. | SAN TELMO | |
| CHACARITA | vs. | BELGRANO | BROWN (ADROGUÉ) | vs. | DEFENSORES | |
| ATLANTA | vs. | DEP. RIESTRA | GUILLERMO BROWN | vs. | SANTAMARINA | |
| MITRE (SGO) | vs. | GIMNASIA (MZA) | ATL RAFAELA | vs. | ALMAGRO | |
| ESTUDIANTES | vs. | ESTUDIANTES RC | IND. RIVADAVIA | vs. | GÜEMES (SGO) | |
| ALVARADO | vs. | TEMPERLEY | BARRACAS CTRAL. | vs. | DEP. MORÓN | |
| TIGRE | vs. | DEP. MAIPÚ | INSTITUTO | vs. | FERRO | |
| QUILMES | vs. | AGROPECUARIO | GIMNASIA (JUJUY) | vs. | TRISTÁN SUÁREZ | |
| FECHA 7 | FECHA 7 | |||||
| LIBRE | vs. | QUILMES | ALL BOYS | vs. | GIMNASIA (JUJUY) | |
| AGROPECUARIO | vs. | TIGRE | TRISTÁN SUÁREZ | vs. | INSTITUTO | |
| DEP. MAIPÚ | vs. | ALVARADO | FERRO | vs. | BARRACAS CTRAL. | |
| TEMPERLEY | vs. | ESTUDIANTES | DEP. MORÓN | vs. | IND. RIVADAVIA | |
| ESTUDIANTES RC | vs. | MITRE (SGO) | GÜEMES (SGO) | vs. | ATL RAFAELA | |
| GIMNASIA (MZA) | vs. | ATLANTA | ALMAGRO | vs. | GUILLERMO BROWN | |
| DEP. RIESTRA | vs. | CHACARITA | SANTAMARINA | vs. | BROWN (ADROGUÉ) | |
| BELGRANO | vs. | NUEVA CHICAGO | DEFENSORES | vs. | SAN MARTÍN (SJ) | |
| SAN MARTÍN (TUC) | vs. | ALMIRANTE BROWN | SAN TELMO | vs. | VILLA DÁLMINE | |
| FECHA 8 | FECHA 8 | |||||
| SAN MARTÍN (TUC) | vs. | LIBRE | SAN TELMO | vs. | ALL BOYS | |
| ALMIRANTE BROWN | vs. | BELGRANO | VILLA DÁLMINE | vs. | DEFENSORES | |
| NUEVA CHICAGO | vs. | DEP. RIESTRA | SAN MARTÍN (SJ) | vs. | SANTAMARINA | |
| CHACARITA | vs. | GIMNASIA (MZA) | BROWN (ADROGUÉ) | vs. | ALMAGRO | |
| ATLANTA | vs. | ESTUDIANTES RC | GUILLERMO BROWN | vs. | GÜEMES (SGO) | |
| MITRE (SGO) | vs. | TEMPERLEY | ATL RAFAELA | vs. | DEP. MORÓN | |
| ESTUDIANTES | vs. | DEP. MAIPÚ | IND. RIVADAVIA | vs. | FERRO | |
| ALVARADO | vs. | AGROPECUARIO | BARRACAS CTRAL. | vs. | TRISTÁN SUÁREZ | |
| TIGRE | vs. | QUILMES | INSTITUTO | vs. | GIMNASIA (JUJUY) | |
| FECHA 9 | FECHA 9 | |||||
| LIBRE | vs. | TIGRE | ALL BOYS | vs. | INSTITUTO | |
| QUILMES | vs. | ALVARADO | GIMNASIA (JUJUY) | vs. | BARRACAS CTRAL. | |
| AGROPECUARIO | vs. | ESTUDIANTES | TRISTÁN SUÁREZ | vs. | IND. RIVADAVIA | |
| DEP. MAIPÚ | vs. | MITRE (SGO) | FERRO | vs. | ATL RAFAELA | |
| TEMPERLEY | vs. | ATLANTA | DEP. MORÓN | vs. | GUILLERMO BROWN | |
| ESTUDIANTES RC | vs. | CHACARITA | GÜEMES (SGO) | vs. | BROWN (ADROGUÉ) | |
| GIMNASIA (MZA) | vs. | NUEVA CHICAGO | ALMAGRO | vs. | SAN MARTÍN (SJ) | |
| DEP. RIESTRA | vs. | ALMIRANTE BROWN | SANTAMARINA | vs. | VILLA DÁLMINE | |
| BELGRANO | vs. | SAN MARTÍN (TUC) | DEFENSORES | vs. | SAN TELMO | |
| FECHA 10 | FECHA 10 | |||||
| BELGRANO | vs. | LIBRE | DEFENSORES | vs. | ALL BOYS | |
| SAN MARTÍN (TUC) | vs. | DEP. RIESTRA | SAN TELMO | vs. | SANTAMARINA | |
| ALMIRANTE BROWN | vs. | GIMNASIA (MZA) | VILLA DÁLMINE | vs. | ALMAGRO | |
| NUEVA CHICAGO | vs. | ESTUDIANTES RC | SAN MARTÍN (SJ) | vs. | GÜEMES (SGO) | |
| CHACARITA | vs. | TEMPERLEY | BROWN (ADROGUÉ) | vs. | DEP. MORÓN | |
| ATLANTA | vs. | DEP. MAIPÚ | GUILLERMO BROWN | vs. | FERRO | |
| MITRE (SGO) | vs. | AGROPECUARIO | ATL RAFAELA | vs. | TRISTÁN SUÁREZ | |
| ESTUDIANTES | vs. | QUILMES | IND. RIVADAVIA | vs. | GIMNASIA (JUJUY) | |
| ALVARADO | vs. | TIGRE | BARRACAS CTRAL. | vs. | INSTITUTO | |
| FECHA 11 | FECHA 11 | |||||
| LIBRE | vs. | ALVARADO | ALL BOYS | vs. | BARRACAS CTRAL. | |
| TIGRE | vs. | ESTUDIANTES | INSTITUTO | vs. | IND. RIVADAVIA | |
| QUILMES | vs. | MITRE (SGO) | GIMNASIA (JUJUY) | vs. | ATL RAFAELA | |
| AGROPECUARIO | vs. | ATLANTA | TRISTÁN SUÁREZ | vs. | GUILLERMO BROWN | |
| DEP. MAIPÚ | vs. | CHACARITA | FERRO | vs. | BROWN (ADROGUÉ) | |
| TEMPERLEY | vs. | NUEVA CHICAGO | DEP. MORÓN | vs. | SAN MARTÍN (SJ) | |
| ESTUDIANTES RC | vs. | ALMIRANTE BROWN | GÜEMES (SGO) | vs. | VILLA DÁLMINE | |
| GIMNASIA (MZA) | vs. | SAN MARTÍN (TUC) | ALMAGRO | vs. | SAN TELMO | |
| DEP. RIESTRA | vs. | BELGRANO | SANTAMARINA | vs. | DEFENSORES | |
| FECHA 12 | FECHA 12 | |||||
| DEP. RIESTRA | vs. | LIBRE | SANTAMARINA | vs. | ALL BOYS | |
| BELGRANO | vs. | GIMNASIA (MZA) | DEFENSORES | vs. | ALMAGRO | |
| SAN MARTÍN (TUC) | vs. | ESTUDIANTES RC | SAN TELMO | vs. | GÜEMES (SGO) | |
| ALMIRANTE BROWN | vs. | TEMPERLEY | VILLA DÁLMINE | vs. | DEP. MORÓN | |
| NUEVA CHICAGO | vs. | DEP. MAIPÚ | SAN MARTÍN (SJ) | vs. | FERRO | |
| CHACARITA | vs. | AGROPECUARIO | BROWN (ADROGUÉ) | vs. | TRISTÁN SUÁREZ | |
| ATLANTA | vs. | QUILMES | GUILLERMO BROWN | vs. | GIMNASIA (JUJUY) | |
| MITRE (SGO) | vs. | TIGRE | ATL RAFAELA | vs. | INSTITUTO | |
| ESTUDIANTES | vs. | ALVARADO | IND. RIVADAVIA | vs. | BARRACAS CTRAL. | |
| FECHA 13 | FECHA 13 | |||||
| LIBRE | vs. | ESTUDIANTES | ALL BOYS | vs. | IND. RIVADAVIA | |
| ALVARADO | vs. | MITRE (SGO) | BARRACAS CTRAL. | vs. | ATL RAFAELA | |
| TIGRE | vs. | ATLANTA | INSTITUTO | vs. | GUILLERMO BROWN | |
| QUILMES | vs. | CHACARITA | GIMNASIA (JUJUY) | vs. | BROWN (ADROGUÉ) | |
| AGROPECUARIO | vs. | NUEVA CHICAGO | TRISTÁN SUÁREZ | vs. | SAN MARTÍN (SJ) | |
| DEP. MAIPÚ | vs. | ALMIRANTE BROWN | FERRO | vs. | VILLA DÁLMINE | |
| TEMPERLEY | vs. | SAN MARTÍN (TUC) | DEP. MORÓN | vs. | SAN TELMO | |
| ESTUDIANTES RC | vs. | BELGRANO | GÜEMES (SGO) | vs. | DEFENSORES | |
| GIMNASIA (MZA) | vs. | DEP. RIESTRA | ALMAGRO | vs. | SANTAMARINA | |
| FECHA 14 | FECHA 14 | |||||
| GIMNASIA (MZA) | vs. | LIBRE | ALMAGRO | vs. | ALL BOYS | |
| DEP. RIESTRA | vs. | ESTUDIANTES RC | SANTAMARINA | vs. | GÜEMES (SGO) | |
| BELGRANO | vs. | TEMPERLEY | DEFENSORES | vs. | DEP. MORÓN | |
| SAN MARTÍN (TUC) | vs. | DEP. MAIPÚ | SAN TELMO | vs. | FERRO | |
| ALMIRANTE BROWN | vs. | AGROPECUARIO | VILLA DÁLMINE | vs. | TRISTÁN SUÁREZ | |
| NUEVA CHICAGO | vs. | QUILMES | SAN MARTÍN (SJ) | vs. | GIMNASIA (JUJUY) | |
| CHACARITA | vs. | TIGRE | BROWN (ADROGUÉ) | vs. | INSTITUTO | |
| ATLANTA | vs. | ALVARADO | GUILLERMO BROWN | vs. | BARRACAS CTRAL. | |
| MITRE (SGO) | vs. | ESTUDIANTES | ATL RAFAELA | vs. | IND. RIVADAVIA | |
| FECHA 15 | FECHA 15 | |||||
| LIBRE | vs. | MITRE (SGO) | ALL BOYS | vs. | ATL RAFAELA | |
| ESTUDIANTES | vs. | ATLANTA | IND. RIVADAVIA | vs. | GUILLERMO BROWN | |
| ALVARADO | vs. | CHACARITA | BARRACAS CTRAL. | vs. | BROWN (ADROGUÉ) | |
| TIGRE | vs. | NUEVA CHICAGO | INSTITUTO | vs. | SAN MARTÍN (SJ) | |
| QUILMES | vs. | ALMIRANTE BROWN | GIMNASIA (JUJUY) | vs. | VILLA DÁLMINE | |
| AGROPECUARIO | vs. | SAN MARTÍN (TUC) | TRISTÁN SUÁREZ | vs. | SAN TELMO | |
| DEP. MAIPÚ | vs. | BELGRANO | FERRO | vs. | DEFENSORES | |
| TEMPERLEY | vs. | DEP. RIESTRA | DEP. MORÓN | vs. | SANTAMARINA | |
| ESTUDIANTES RC | vs. | GIMNASIA (MZA) | GÜEMES (SGO) | vs. | ALMAGRO | |
| FECHA 16 | FECHA 16 | |||||
| ESTUDIANTES RC | vs. | LIBRE | GÜEMES (SGO) | vs. | ALL BOYS | |
| GIMNASIA (MZA) | vs. | TEMPERLEY | ALMAGRO | vs. | DEP. MORÓN | |
| DEP. RIESTRA | vs. | DEP. MAIPÚ | SANTAMARINA | vs. | FERRO | |
| BELGRANO | vs. | AGROPECUARIO | DEFENSORES | vs. | TRISTÁN SUÁREZ | |
| SAN MARTÍN (TUC) | vs. | QUILMES | SAN TELMO | vs. | GIMNASIA (JUJUY) | |
| ALMIRANTE BROWN | vs. | TIGRE | VILLA DÁLMINE | vs. | INSTITUTO | |
| NUEVA CHICAGO | vs. | ALVARADO | SAN MARTÍN (SJ) | vs. | BARRACAS CTRAL. | |
| CHACARITA | vs. | ESTUDIANTES | BROWN (ADROGUÉ) | vs. | IND. RIVADAVIA | |
| ATLANTA | vs. | MITRE (SGO) | GUILLERMO BROWN | vs. | ATL RAFAELA | |
| FECHA 17 | FECHA 17 | |||||
| LIBRE | vs. | ATLANTA | ALL BOYS | vs. | GUILLERMO BROWN | |
| MITRE (SGO) | vs. | CHACARITA | ATL RAFAELA | vs. | BROWN (ADROGUÉ) | |
| ESTUDIANTES | vs. | NUEVA CHICAGO | IND. RIVADAVIA | vs. | SAN MARTÍN (SJ) | |
| ALVARADO | vs. | ALMIRANTE BROWN | BARRACAS CTRAL. | vs. | VILLA DÁLMINE | |
| TIGRE | vs. | SAN MARTÍN (TUC) | INSTITUTO | vs. | SAN TELMO | |
| QUILMES | vs. | BELGRANO | GIMNASIA (JUJUY) | vs. | DEFENSORES | |
| AGROPECUARIO | vs. | DEP. RIESTRA | TRISTÁN SUÁREZ | vs. | SANTAMARINA | |
| DEP. MAIPÚ | vs. | GIMNASIA (MZA) | FERRO | vs. | ALMAGRO | |
| TEMPERLEY | vs. | ESTUDIANTES RC | DEP. MORÓN | vs. | GÜEMES (SGO) |
